Reporter

Part:BBa_K1459008:Design

Designed by: Grzegorz Ścibisz   Group: iGEM14_Warsaw   (2014-10-07)


pmrC-GFP-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1054


Design Notes

We had to conduct directed mutagenesis to remove XbaI site from pmrC sequence.


Source

We obtained pmrC-GFP sequence thanks to kindness of prof. he from university of Chicago.

References